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aureum. No 13C18-C22:six was made inside of a. limacinum beneath the same conditions, indicating that DHA is not really made by the ELO/DES pathway within a. limacinum (Fig. 1e). It absolutely was claimed, nevertheless, that many genes linked to the ELO/DES pathway are existing inside the genome of the. limacinum7, and therefore, a tracing experiment using secure isotope-labeled PA (d31–C16:0) was performed to pick which phase of the ELO/DES pathway in the. limacinum will not perform in vivo. We found that d31–C18:0 and d29–C18:1 ended up created in Parietichytrium sp. and T. aureum (WT) when d31–C16:0 was added to each culture; on the other hand, in A. limacinum d29–C18:1 could not be detected whatsoever, Even though a small level of d31–C16:0 was converted to d31–C18:0 (Supplementary Fig. S3b, c). This end result indicates that the machinery for that conversion of C16:0 to C18:one is not thoroughly practical in A. limacinum, and in particular, Δ9DES, that's chargeable for the conversion of C18:0 to C18:one, doesn't perform in vivo. The reality that the C16ELO homolog exists during the A. limacinum draft genome database however the Δ9DES homolog does not, that is according to the outcome acquired in this analyze. Hence, we conclude which the ELO/DES pathway will not operate in vivo due at least to The shortage of Δ9DES operate Which DHA is exclusively produced in the PUFA-S pathway in A. limacinum.
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